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#Ecoli

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#FYI #USA #MicrobeTV #TWiV #ThisWeekInVirology #DanielGriffin #VincentRacaniello #measles #vaccinate #vaccines #ecoli #covid19 #covid #sarscov2 #RSV

TWiV 1210: Clinical update with Dr. Daniel Griffin week 17.04.2025

56000 confirmed fired federal workers. More than third of the cuts target health services, aiming to deliberately disrupt disease control and research as well as national and global human health services. #fascism #stopfascism #trump #musk #hostiletakeover

youtube.com/watch?v=BsQ_PprPbH4

"A deadly E. coli outbreak linked to romaine lettuce ripped through 15 states at the end of last year, but the Food and Drug Administration didn’t publicly disclose it, according to a report.

The outbreak was linked to the death of one person and hospitalized 36 people in November, including a 9-year-old boy who nearly died of kidney failure."

~ Rhian Lubin

#EColi #Trump #FDA #HHS #healthcare #medicine
/1

independent.co.uk/news/health/

The Independent · Deadly E. coli outbreak linked to lettuce hit 15 states. The government chose to keep it secretBy Rhian Lubin

So, this probably explains why the @fdarecall_bot stopped working... #FoodSafety just flew out the window!!! Thanks #Trump & #DOGE! Keeping us safe...NOT!

A deadly #EColi outbreak hit 15 states, but the #FDA chose not to publicize it

The outbreak linked to #RomaineLettuce killed one person and sickened at least 88 more, including a 9-year-old boy who nearly died of kidney failure.

April 17, 2025, 5:00 AM EDT / Updated April 17, 2025, 2:01 PM EDT
By Suzy Khimm

"An E. coli outbreak linked to romaine lettuce ripped across 15 states in November, sickening dozens of people, including a 9-year-old boy in Indiana who nearly died of kidney failure and a 57-year-old Missouri woman who fell ill after attending a funeral lunch. One person died.

"But chances are you haven’t heard about it.

"The Food and Drug Administration indicated in February that it had closed the investigation without publicly detailing what had happened — or which companies were responsible for growing and processing the contaminated #lettuce."

Read more:
nbcnews.com/news/us-news/ecoli

NBC News · A deadly E. coli outbreak hit 15 states, but the FDA chose not to make the details publicBy Suzy Khimm

Trodde att man gjorde insulin från grispancreas, tror det var så man började iaf. Så överhörde jag en föreläsning min dotter tittade på. Tydligen kan man mippla m DNAt i ecolibakterier o få dem at producera insulin. Typ så förstod jag det, får läsa på. Men ändå. Magi!

bioRxiv · 30S-seq redefines the bacterial Ribosome Binding SiteThe translation initiation step is rate limiting for the efficiency of gene expression in all organisms. However, the mechanism of ribosome recruitment to mRNA start sites strikingly differs between eukaryotes and prokaryotes. The eukaryotic small (40S) ribosomal subunit binds 5' end caps and scans for the start codon while the bacterial small (30S) subunit directly binds to the Shine-Dalgarno (SD) motif close to the initiation site. Pioneer studies have shown rare 30S loading events further upstream within 5' untranslated regions (5'UTRs), at ribosome standby sites (1-3). Together with the frequent occurrence of long bacterial mRNA 5'UTRs and degenerated SD sequences, this indicates that the 30S subunit might bind upstream of the SD more commonly than currently thought. We therefore developed 30S-seq to map 30S-mRNA interactions in a bacterial transcriptome (Escherichia coli), inspired by translation complex profile sequencing (TCP-seq) previously used in eukaryotes (4,5). Our results provide new and unsuspected insights into the behaviour of 30S and 70S complexes during the canonical translation initiation process. Notably, 30S subunits are recruited upstream of the start codon, primed to receive the SD released by the departing 70S ribosome. Remarkably, we also find hundreds of non-canonical 30S binding sites within mRNA 5'UTRs, sometimes over 100 nucleotides upstream of the start region. We validated several of these upstream ribosome binding sites, and demonstrated their strong impact on gene expression. Thus, even in bacteria, ribosomes frequently bind mRNAs outside of the start region to initiate translation, challenging the classic ribosome binding site model. ### Competing Interest Statement The authors have declared no competing interest.

We are excited to welcome back Dr. Ali Hassanali from the Abdus Salam International Centre for Theoretical Physics (ICTP) to Enabla with his second lecture🎉 This time, Dr. Hassanali explores the intersection of biology and physics by analyzing E. coli as a model system to understand biological efficiency. He dives into critical topics such as the concentration of proteins and water molecules within the cell, the effects of particle crowding, and the kinetics of diffusion in crowded environments. Furthermore, he sheds light on the energy dynamics that enable efficient biological operations, all while considering the nanoscale interactions vital to cellular functions.

🎥 This #OpenAccess lecture is now available for free! Join the Enabla community in discussing the lecture and engage directly with Dr. Hassanali using our in-time discussions feature to explore your questions about protein dynamics and biophysics: enabla.com/pub/1164/about

These are the 20 most-studied — majority ignored
Model microbes such as Escherichia coli hog scientists’ attention, leaving most known bacteria with few publications devoted to them.
Study of over 43,000 bacterial species found dominates with 21% of all publications, followed by human pathogens like aureus. Microbes crucial for human health and Earth's ecosystems remain largely unexplored, Univ of Michigan biologist Paul Jensen reported.
nature.com/articles/d41586-025

www.nature.comThese are the 20 most-studied bacteria — the majority have been ignoredModel microbes such as Escherichia coli hog scientists’ attention, leaving most known bacteria with few publications devoted to them.